Request QuoteCatalog Number: xP472945BYTSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP472945BYTYeast1mgQuote
EP472945BYTE. coli1mgQuote
BP472945BYTBaculovirus200ugQuote
MP472945BYTMammalian Cell200ugQuote

Protein Information

SpeciesAnaeromyxobacter sp. (strain K)
UniProt IDB4UAN0
Gene NameleuC; Locus:AnaeK_1898
Protein Name
Region Expressed1-480
Expression Tag6xHis
Purity>90%
AA SequenceMSKRSDGSQPRTIVDKVWDAHVVRAETPDAPAILYIDLHLVHEVTSPQAFTVLRERGLKL RRPERTLATMDHSIPTLPRGADGRWPFVDAQAAAQVSQMERNCADFGVELHGLGDDAQGV VHVFGPEMGATQPGMTVVCGDSHTATHGAFGALAFGIGTSEVGHVLASQCLLQRRPRTLA VRVDGQLGPGLSAKDLILAIIAKLGVGGGTGHVIEYLGPAVRALSMEGRMTLCNMSIEAG ARAGLVAPDDTTFEWLAGRPRAPKGAAWDEAVARWRALPSDEGATYDRELRLDAAALEPM ITFGTNPGQGIAVTGLVPDPVAERDASARATLEAALRYMGLVPGKPIAGQKVDVVFIGSC TNGRIEDMREAARVLKGRKVRTRTLVVPGSHRVKKDAEAEGIDRIVREAGAEWREPGCSM CIAMNGDNIQAGQYCVSTSNRNFEGRQGPGGRTLLASPLTAAAAAVTGAVADPRRVLEGR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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