Request QuoteCatalog Number: xP472877SWVSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP472877SWVYeast1mgQuote
EP472877SWVE. coli1mgQuote
BP472877SWVBaculovirus200ugQuote
MP472877SWVMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella schwarzengrund (strain CVM19633)
UniProt IDB4TWV9
Gene NameleuC; Locus:SeSA_A0127
Protein Name
Region Expressed1-466
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFAT MDHNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLP GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITA KDIVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTF NYVKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVTLRAEEIAPQVTWGTNPGQVISV TDIIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAE VAKGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSIK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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