Request QuoteCatalog Number: xP465160RLVSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP465160RLVYeast1mgQuote
EP465160RLVE. coli1mgQuote
BP465160RLVBaculovirus200ugQuote
MP465160RLVMammalian Cell200ugQuote

Protein Information

SpeciesRhodopseudomonas palustris (strain TIE-1)
UniProt IDB3Q851
Gene NameleuC; Locus:Rpal_0240
Protein Name
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMSAKPTTLYDKIWNDHLVHEAEDGTCLLYIDRHLVHEVTSPQAFEGLRTAGRKVHAPEKT LAVVDHNVPTTDRSKPNPDPESAEQIAALAENARDFGITYYNEFDKRQGVVHVIGPEQGF TLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMRVTVDGQLPDG VTAKDVILAIIGEIGTAGGTGYVLEYAGDAIRSLSMEGRMTVCNMSIEGGARAGLIAPDA KAYEFLKGRPLAPKGEAWDAALRYWETLRSDDGAHFDHELKLDAAALPPIVTWGTSPEDV ISVTGRVPNPADIADEAKRLSKERALAYMGLTPGTKITDIKIDRMFIGSCTNGRIEDLRA AAKVAEGKTVNANVNAIIVPGSGLVKEQAEAEGLDKIFIKAGFEWREPGCSMCLAMNPDK LAPEERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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