Request QuoteCatalog Number: xP463194RKLSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP463194RKLYeast1mgQuote
EP463194RKLE. coli1mgQuote
BP463194RKLBaculovirus200ugQuote
MP463194RKLMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium etli (strain CIAT 652)
UniProt IDB3PS18
Gene NameleuD; Locus:RHECIAT_CH0004381
Protein Name
Region Expressed1-202
Expression Tag6xHis
Purity>90%
AA SequenceMDKFVKLTGVAAPLPVVNIDTDMIIPKDYLKTIKRTGLGKGLFAEARYNEDGSENPDFVL NKPAYRDAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIK VSQEDLDKLMDDASRGSNAILTVDLENLEITGPDGGSIKFDLDAFKRHCLLNGLDDIGLT MEKGKAIDEFEKKNAASHPWAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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