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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP490286HUEYeast1mgQuote
EP490286HUEE. coli1mgQuote
BP490286HUEBaculovirus200ugQuote
MP490286HUEMammalian Cell200ugQuote

Protein Information

SpeciesHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
UniProt IDB8CX22
Gene NameleuC; Locus:Hore_10870
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-420
Expression Tag6xHis
Purity>90%
AA SequenceMGMTMIEKIIAAHTNRDKVKPGDIVNARIDFVLGNDVTTPVAVKEFNKIGIDEVFDQDRI AIVPDHFTPNKDIKSAEQCKFIREFARDKGIHNYFEIGEMGIEHCLLPEKGLALPGEIII GADSHTCTYGALGAFATGVGSTDMAAAMATGYTWFKVPSTIQFVYNGELKPWVSGKDLIL YTIGDIGVDGALYRAMEFTGETIENLSMDNRMTISNMAIEAGGKCGLIAPDEKTFKYLEN RAQRDYTPCYSDDDAEYEKVIEYDVSDIEPQVAFPHLPENTRPISEEGDIKIDQAVIGSC TNGRMEDLRIAAGILKEKQVHHDVRCIVIPGTQDIYKQALKEGLIEIFIEAGAAVSTPTC GPCLGGHMGILARGEKAIATTNRNFVGRMGHPESEVYLANPAVAAASAIKGYIAHPEEVL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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