Request QuoteCatalog Number: xP457315DQYSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP457315DQYYeast1mgQuote
EP457315DQYE. coli1mgQuote
BP457315DQYBaculovirus200ugQuote
MP457315DQYMammalian Cell200ugQuote

Protein Information

SpeciesCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
UniProt IDB3PFN3
Gene NameleuD; Locus:CJA_1745
Protein Name
Region Expressed1-215
Expression Tag6xHis
Purity>90%
AA SequenceMKSFTVLDGLAAPMDRANVDTDMIIPKQFLKSIKRTGFGKNLFDELRYLDEGKPDQSCEG RPLNTEFPLNFPRYQDASVLLARENFGCGSSREHAPWALDDYGFRSVIAPSFADIFFNNC FKNGLLPIMLDEKIVDQLFREMYASEGYRLNIDLAEQTVTTPSGESFGFEIDAFRKHCLL NGLDDIGLTLEKADKIRAYETRRRAEAPWLFDVVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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