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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP459276DQYYeast1mgQuote
EP459276DQYE. coli1mgQuote
BP459276DQYBaculovirus200ugQuote
MP459276DQYMammalian Cell200ugQuote

Protein Information

SpeciesCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
UniProt IDB3PFN2
Gene NameleuC; Locus:CJA_1744
Protein Name
Region Expressed1-473
Expression Tag6xHis
Purity>90%
AA SequenceMVAKTLYDKLWDAHLVHESDDGSALIYIDRHIVHEVTSPQAFDGLRMAGRKPWRVDSILA TPDHNVPTTQKERAHGVSGIQDPVSLIQVQTLDDNCDEFGITEFKINDSRQGIVHVVGPE SGACLPGMTIVCGDSHTATNGALGALAHGIGTSEVEHVMATQCLVAKKMKNMLIKVDGTL GLGVTPKDVVLAIIAKIGTAGGTGYAMEFGGQVFRDMSMEGRLTVCNMAIEAGARAGMVA VDQTTIDYVKGRPYAPSGDVWEQAVANWRELVSDEGAHFDAVIEIDGASIRPQVSWGTSP EMVVSVEDKVPDPANEPDPVKRKDMIRALEYMGLQANQPITSIYVDRVFIGSCTNSRIED IRAAAEVVKGKQKAANVKEAIVVPGSGVVKAQAEAEGLDKIFTAAGIEWREPGCSMCLAM NADKLGAGEHCASTSNRNFEGRQGYGGRTHLVSPAMAAAAAIAGHFVDVRSFN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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