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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP457237AXIYeast1mgQuote
EP457237AXIE. coli1mgQuote
BP457237AXIBaculovirus200ugQuote
MP457237AXIMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus pleuropneumoniae serotype 7 (strain AP76)
UniProt IDB3GZY1
Gene NameleuC; Locus:APP7_0141
Protein Name
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMAKTLYEKLFDAHVVYEAAGETPILYINRHLIHEVTSPQAFDGLRVAGRQVRQIGKTFGT MDHSISTQVRDVNKLEGQAKIQVLELAKNCEANGISLFDMQTKEQGIVHVMGPEQGLTLP GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQARAKSMKVEVRGKVNPGITA KDIVLAIIGKTTMAGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIEFGAKAGLVAPDETTF AYLKGRPHAPKGKDWDDAVEYWKTLKSDDDAVFDSVVVLEAKDIAPQVTWGTNPGQVIGI DQVVPNPQEMADPVTKASAEKALAYIGLDANTDMKNIPVDQVFIGSCTNSRIEDLRAAAA VMKGRKKADNVKRVLVVPGSGLVKEQAEKEGLDKIFIEAGAEWRNPGCSMCLGMNDDRLG EWERCASTSNRNFEGRQGRNGRTHLVSPAMAAAAAMFGKFVDIRHVELN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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