Request QuoteCatalog Number: xP493093MTFSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP493093MTFYeast1mgQuote
EP493093MTFE. coli1mgQuote
BP493093MTFBaculovirus200ugQuote
MP493093MTFMammalian Cell200ugQuote

Protein Information

SpeciesMethylocella silvestris (strain BL2 / DSM 15510 / NCIMB 13906)
UniProt IDB8ENI5
Gene NameleuD; Locus:Msil_1147
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-201
Expression Tag6xHis
Purity>90%
AA SequenceMDKFTSLTGVAAPLKITNVDTDMLIPKQYLKTIKRTGLGSGLFAELRYREDGSENPDFVL NKPAYRKASILVAGDNFGCGSSREHAPWALLDFGIRCVIATSFADIFYNNCFKNGILPIV VSPEELEKLMDDAERGANATLSIDLDAQEIRGPDGGRIRFEIDPFRKHCLLNGLDDIGLT LEKGVHIDAHEQKIEGRRSWL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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