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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP489812BSUYeast1mgQuote
EP489812BSUE. coli1mgQuote
BP489812BSUBaculovirus200ugQuote
MP489812BSUMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium animalis subsp. lactis (strain AD011)
UniProt IDB8DVN1
Gene NameleuD; Locus:BLA_0230
Protein Name3-isopropylmalate dehydratase small subunit
Region Expressed1-226
Expression Tag6xHis
Purity>90%
AA SequenceMEKLTTITATGVPLRRSNVDTDQIIPAVFLKRVTRTGFDDALFYAWRRDPNFVLNQPEYR DGRILVAGPDFGIGSSREHAVWALHDYGFRVVIASRFADIFYGNTAKNGVLAAIMPQESI ELIWKMLEEEPGRQMTVDLETRTVTIDDVVLPFEVNDYVRWRLMNGYDDIDLTLQHEDDI AAYERMRAEKFPFKPKTLPAITEEAETVESAREPEAVEWAGPLADR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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