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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488515BSUYeast1mgQuote
EP488515BSUE. coli1mgQuote
BP488515BSUBaculovirus200ugQuote
MP488515BSUMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium animalis subsp. lactis (strain AD011)
UniProt IDB8DVN0
Gene NameleuC; Locus:BLA_0229
Protein Name3-isopropylmalate dehydratase large subunit
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMGMTLAEKVWADHLVRKGSDGAPDLLYIDLMLMHEVTSPQAFEGLRLAGRTPRHLDQLIA TEDHNTPTVDIDKPNPDETSATQLTTLEKNCREFGVRLHPLGDADQGIVHAFAPMLGLTQ PGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQTLSLKPFKTMAINIEGDLPAGVS AKDIILAIIAKIGTGGGQGYVLEYRGRAIEQLSMDARMTICNMSIEAGARAGMIAPDETT FAYLKGRPHAPKGEMWDQAVEYWKTLRTDDDAVFDAEVTLRAEDLEPFVTWGTNPGQGVA ISGVVPAPDDFADATERSAAERALDYMGLEPGTPIRDIPVDTVFIGSCTNGRIEDLRIAA EIMKGHKKADSIHRVLVVPASSRIRLQAEREGLDRIFKDFGAEWRNAGCSMCLGMNPDKL VAHERSISTSNRNFEGRQGKGSRTHLASPAVAAATAIRGTISTPADL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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