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Recombinant 3-isopropylmalate dehydratase small subunit (leuD)

Recombinant 3-isopropylmalate dehydratase small subunit (leuD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP465406EYZYeast1mgQuote
EP465406EYZE. coli1mgQuote
BP465406EYZBaculovirus200ugQuote
MP465406EYZMammalian Cell200ugQuote

Protein Information

SpeciesProteus mirabilis (strain HI4320)
UniProt IDB4F197
Gene NameleuD; Locus:PMI2087
Protein Name
Region Expressed1-200
Expression Tag6xHis
Purity>90%
AA SequenceMNKFTQHTGIPVPLDAANVDTDAIIPKQFLQKVTRTGFGKHLFHDWRFLDDEGTQPNPSF ILNQPIYQDASILLARENFGCGSSREHAPWALTDYGFHAVIAPSFADIFYGNSFNNQLLP IKLSDEQVTQLFNWVTEHAGTPITVDLVAQQVIAGELCFSFEIDAFRRHCMIEGLDSIGL TLQHQAEISAYEAKQPAFMR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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