Request QuoteCatalog Number: xP461523EYZSize: 0.2-1mg

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Recombinant 3-isopropylmalate dehydratase large subunit (leuC)

Recombinant 3-isopropylmalate dehydratase large subunit (leuC) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP461523EYZYeast1mgQuote
EP461523EYZE. coli1mgQuote
BP461523EYZBaculovirus200ugQuote
MP461523EYZMammalian Cell200ugQuote

Protein Information

SpeciesProteus mirabilis (strain HI4320)
UniProt IDB4F196
Gene NameleuC; Locus:PMI2086
Protein Name
Region Expressed1-469
Expression Tag6xHis
Purity>90%
AA SequenceMGKTLYQKIYDAHVVREVANETPIIYIDRHLVHEVTSPQAFDGLRTKGRAVRQPNKTFAT MDHNVSTQTKDINACGDMARIQMQELMKNCQEFGVTLYDLNHPYQGIVHVMGPEQGLTLP GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQSRAKTLKIEVQGKTANGITA KDIVLAIIGKLGSAGGTGYIIEFTGEAIEALSMEGRMTLCNMAIEMGAKAGLVAPDETTF AYLKGRQFAPQAPLWDEAVAYWKTLKSDEDAIFDATVTINAAEIAPQVTWGTNPGQVIAI DQIVPRLNSFNDPVERASAEKALAYMGLTEGVNLTDITIDKVFIGSCTNSRIEDLRAAAK IAKGHKVASHVQAIVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNP GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIHGHFADIRTFATVS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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