The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Ser-Leu-Pro.
As measured by its binding ability in a functional ELISA, immobilized IL1F10 at 1 ug/ml (100 ul/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 ug/ml.
IL1F10 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to quick spin followed by reconstitution of IL1F10 in PBS to a concentration no less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized IL1F10 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL1F10 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please avoid multiple freeze-thaw cycles.
Interleukin-1 family member 10, IL-1F10, FIL1 theta, Interleukin-1 HY2, IL-1HY2, Interleukin-1 theta, IL-1 theta, IL1F10, FIL1T, IL1HY2, FKSG75, MGC119831, MGC119832, MGC119833, FIL1-theta.
