SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI.
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is < 0.5ng/ml, corresponding to a specific activity of 2,000,000 units/mg.
The Mouse IL-33 was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH-7.4, 150mM sodium chloride, 1mM EDTA and 2mM b-ME.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized IL-33 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized IL-33 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL-33 should be stored at 4oC between 2-7 days and for future use below -18oC.
Please avoid multiple freeze-thaw cycles.
Interleukin 33, DVS27, NF-HEV, NKHEV, C9orf26, Interleukin-1 family member 11, IL- 1F11, Nuclear factor from high endothelial venules, Il-33, Il1f11, 9230117N10Rik, Il33.
