Direct ELISA, Western Blot, Immuneprecipitation.
This antibody recognizes the entire IL-12 molecule and has no reactivity to denatured p40 or p35.
1mg/ml in PBS (after reconstitution).
Reconstitute with sterile H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.
Antibody is shipped lyophilized at ambient temperature.
In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Rat Anti Mouse Monoclonal.