The sequence of the first five N-terminal amino acids of IL-12 was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.
IL-12 activity was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/ml corresponding to a specific activity of 2-3.3MU/mg.
IL-12 was lyophilized from a 0.2um filtered solution (0.5mg/ml) in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized IL-12 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
CHO (Chinese Hamster Ovarian Cells).
Lyophilized IL-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IL-12 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid multiple freeze-thaw cycles.
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
