Insulin-Like Growth Factor Binding Protein-3 Human Recombinant
IGFBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton.
IGFBP-3 is purified by proprietary chromatographic techniques.
Accession
Amino acid sequence
GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK.
Biological Activity
The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 200ng/ml corresponding to a specific activity of 5,000U/mg in the presence of 15ng/ml of Human IGF-II.
Formulation
Lyophilized from a 0.2um filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Source
Stability
Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC.
Upon reconstitution IGF-BP 3 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Growth-hormone-dependant binding protein, IBP3, BP-53, IGFBP-3.
