$148.20Catalog Number: PYT300-25ugSize: 25ug

IGFBP 3 Human

Insulin-Like Growth Factor Binding Protein-3 Human Recombinant

IGFBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 2x264 amino acids and having a molecular mass of 28806 Dalton.
IGFBP-3 is purified by proprietary chromatographic techniques.

Accession

P17936

Amino acid sequence

GASSGGLGPV VRCEPCDARA LAQCAPPPAV CAELVREPGC GCCLTCALSE GQPCGIYTER CGSGLRCQPS PDEARPLQAL LDGRGLCVNA SAVSRLRAYL LPAPPAPGNA SESEEDRSAG EVESPSVSST HRVSDPKFHP LHSKIIIIKK GHAKDSQRYK VDYESQSTDT QNFSSESKRE TEYGPCRREM EDTLNHLKFL NVLSPRGVHI PNCDKKGFYK KKQCRPSKGR KRGFCWCVDK YGQPLPGYTT KGKEDVHCYS MQSK.

Biological Activity

The ED50, calculated by by its ability to inhibit IGF-II induced proliferation of MCF-7 is < 200ng/ml corresponding to a specific activity of 5,000U/mg in the presence of 15ng/ml of Human IGF-II.

Formulation

Lyophilized from a 0.2um filtered concentrated (0.5mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized IBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC.
Upon reconstitution IGF-BP 3 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Growth-hormone-dependant binding protein, IBP3, BP-53, IGFBP-3.

Quantity

$148.20

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