$558.60Catalog Number: PYT299-10ugSize: 10ug

IGFBP 1 Human

Insulin-Like Growth Factor Binding Protein-1 Human

IGFBP1 native Human is a single, glycosylated, polypeptide chain containing 218 amino acids and having a molecular mass of 25.3kDa. IGFBP1 is purified by proprietary chromatographic techniques.

Accession

P08833

Biological Activity

Inhibition of IGF-I stimulated proliferation of NUC-1 cells: IC50 ~ 100 ng/ml Inhibition of serum-induced stimulation of DNA synthesis in chick embryo fibroblast primary cultures: IC50 (1% serum) ~300 ng/ml corresponding to a Specific Activity of 3,334IU/mg.

Formulation

The protein was lyophilized with 10mM HCL.

Intorduction

IGFBP1 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized IBP-1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Human amniotic fluid

Stability

Lyophilized IGFBP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC.
Upon reconstitution IGF-BP1 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

IBP-1, IGF-Binding Protein 1, AFBP, PP12, IGF-BP25, hIGFBP-1, IGFBP-1.

Quantity

$558.60

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