$90.00Catalog Number: PYT518-20ugSize: 20ug

IGF1 Human Des1-3

Insulin-Like Growth Factor 1 Des (1-3) Human Recombinant

IGF-I Des(1-3) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7372 Dalton.
IGF-1 Des1-3 is purified by proprietary chromatographic techniques.

Accession

P01343

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Leu-Cys-Gly-Ala.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 1ng/ml, corresponding to a specific activity of 1MIU/mg.For most in-vitro applications, IGF-I des1-3 exerts its biological activity in the concentration range of 0.2-20 ng/ml corresponding to a specific activity of 50,000IU-5MIU/mg.

Formulation

The protein was lyophilized after extensive dialysis against 50mM acetic acid buffer.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized IGF-I Des(1-3) in 100mM Acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized IGF-I des(1-3) although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IGF1 des-1-3 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF, Des(1-3), Des1-3, Des 1-3, Des (1-3), IGF-1 (4-70).

Quantity

$90.00

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