The sequence of the first twelve N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln-Thr-His-Ser-Leu-Gly-Ser-Arg.
The specific activity was determined by a quantitative gene report bioassay using human Type I interferon-sensitive cells. Compared with bacterial derived recombinant IFN-a 2a, assayed in the same conditions, plant derived IFN-alpha 2a showed 6.1x108 Units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 99.0% as determined by both
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution IFN-alpha 2a should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-α 2a.
