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Recombinant 3'-hydroxymethylcephem-O-carbamoyltransferase (cmcH)

Recombinant 3'-hydroxymethylcephem-O-carbamoyltransferase (cmcH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528290FOAYeast1mgQuote
EP528290FOAE. coli1mgQuote
BP528290FOABaculovirus200ugQuote
MP528290FOAMammalian Cell200ugQuote

Protein Information

SpeciesStreptomyces clavuligerus
UniProt IDO85728
Gene NamecmcH
Protein Name3'-hydroxymethylcephem-O-carbamoyltransferase
Region Expressed1-521
Expression Tag6xHis
Purity>90%
AA SequenceMLVVAFKPGHDGAVAAIDDRRLLYSLESEKDSRPRYSTLLPTTFLDIAERLGAIPDVVAL GGWADLRPRGVVYTGAGYEGTQEPTVTTSRFFGKEVKFFTSTHERSHIYMALGMAPKDGA PLKSVLVWEGDVGAFYLVDSEHRIIRTIPVMTGPGARYSFLFGLADPTFPDTGGKPRLND AGKLMALAAFGDSADASPDIRHVVERVLKQDSMYPAPKAEYRDSVLHNAGVESEECKIAA ALLTERLFETFAEVARRELPEGTPLYISGGCGLNCDWNSQWAQLGHFSSVFVAPCTNDSG SALGTAIDALTTFTGDPHIDWNVYSGLEFVHDTRPDPARWESVPLDHAALSSALAAGRVV AWVQGRWEIGPRALGNRSLLAEAFSSASRDRLNTVKMREDYRPIAPVCRVEDLGKVFHED FEDPHMLYFRRVREESGVRAVTHVDGSARCQTVTGTSNPELHRLLSVFAQGQGLGVLCNT SLNFNGDGFINRMSDLVRYCEWREIQDMVVGDTWYRRIAGS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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