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Recombinant Hydroxyethylthiazole kinase (thiM)

Recombinant Hydroxyethylthiazole kinase (thiM) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513305ENUYeast1mgQuote
EP513305ENUE. coli1mgQuote
BP513305ENUBaculovirus200ugQuote
MP513305ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZSI8
Gene NamethiM; Locus:BWG_1890
Protein NameHydroxyethylthiazole kinase
Region Expressed1-262
Expression Tag6xHis
Purity>90%
AA SequenceMQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF AAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVAVGALDYRRHFCHELLSFK PAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDG HRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE GPGSFVPHFLDALWQLTQEVQA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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