Request QuoteCatalog Number: xP529463DSBSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP529463DSBYeast1mgQuote
EP529463DSBE. coli1mgQuote
BP529463DSBBaculovirus200ugQuote
MP529463DSBMammalian Cell200ugQuote

Protein Information

SpeciesChlamydia trachomatis (strain D/UW-3/Cx)
UniProt IDO84060
Gene NameispG; aka: gcpE; Locus:CT_057
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-602
Expression Tag6xHis
Purity>90%
AA SequenceMATPCIQNAFRRKTLPVRIGDLFVGSEHSIKIQSMTTTATTDVDGTVRQICALQEWGCDI VRVTVQGLREVHACEHIKDRLIQQNISIPLVADIHFFPQAAIHVVDCVDKVRINPGNYVD KRNMFTGKIYSDEQYAHSLEHLMNKFSPLVEKCKRLGKAMRIGVNHGSLSERVTQRYGNT IEGMVYSALEYAEVCVAMDYHDVIFSMKSSNPKVMVAAYRSLAYELDQREWSYPLHLGVT EAGSGTAGIVKSAVGIGTLLSEGLGDTIRCSLTGSPINEIPICIDLLKQTTELSERWGEA DNPFAIHSSKQLGTRNTLNTPPWGNVYGLLINLTDVQLLTAEPIELLQCLGIDTTTGKID PTTPEGVVVPKAMRSSPIVSEIEKHLLVFNKEDVPILNPMNEEEWLSEETLSAPFVYFEV TDIHTARRFFSLRQHSTQPVCLSFSLDPHLSKNEAIIDLSARLGALLLDGLGSCVLLDFV DIKLSRTLGFLILQSANIRSVTVEYVSCPGCGRTLFDLLAVSQRIRERTQHLPGGLKIAV MGCIVNGPGEMADADFGYVGSKPGMIDLYVKHKCVKSCIPIENAEEELVQLLKEHGVWKE PE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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