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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511185ESQYeast1mgQuote
EP511185ESQE. coli1mgQuote
BP511185ESQBaculovirus200ugQuote
MP511185ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DBH4
Gene NameispG; Locus:PC1_3014
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-373
Expression Tag6xHis
Purity>90%
AA SequenceMHNAAPITRRKSKRIYVGKVPVGDGAPIAVQSMTNTRTTDVEATVNQIRSLERVGVDIVR VSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRS VVDCARYNNIPIRIGVNGGSLEKDLQEKYGEPTPEALLESAMRHVDILDRLNFDQFKVSV KASDVFLAVQSYRLLAARIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL AADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV SIIGCVVNGPGEALVSTIGVTGGHNKSGFYEDGVRQRERFDNEQMIDQLEAKIRAKASMM DENQRITVNLVDK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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