Request QuoteCatalog Number: xP508023BWLSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)

Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP508023BWLYeast1mgQuote
EP508023BWLE. coli1mgQuote
BP508023BWLBaculovirus200ugQuote
MP508023BWLMammalian Cell200ugQuote

Protein Information

SpeciesBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
UniProt IDC0ZL73
Gene NameispH; Locus:BBR47_49230
Protein Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region Expressed1-311
Expression Tag6xHis
Purity>90%
AA SequenceMEVIKISPRGYCYGVVDAMVLALRTAQNFDLPRPIHILGMIVHNAHVVEAFEKQGIKTLD GEDRLALLDQVQEGTIIFTAHGVSPEVRKKARDKGLTVVDATCPDVTKTHDLIREKVAEG YHVLYIGKKGHPEPEGAMGIAPDHVHLVQKLEELEALDLPTDKLIVTNQTTMSQWDVKHL MDAILKRFPGVEVHNEICLATQVRQEAVAEQVGEADLCIVVGDPRSNNSNRLAQVSEEIA DVPSYRIADLFELDIEWLRGKKNVAVTSGASTPTPLTKEVITFLEQFDEFNPATWEKKRT VNMAKILPTVK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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