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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP499679BWTYeast1mgQuote
EP499679BWTE. coli1mgQuote
BP499679BWTBaculovirus200ugQuote
MP499679BWTMammalian Cell200ugQuote

Protein Information

SpeciesBrucella melitensis biotype 2 (strain ATCC 23457)
UniProt IDC0RF32
Gene NameispG; Locus:BMEA_A1828
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-420
Expression Tag6xHis
Purity>90%
AA SequenceMSSETVSYFSHPFPRRQSVGVSVGGVIVGGSAPVVVQSMTNTDTADVDSTVAQVAALHRA GSEIVRITVDRDESAAAVPKIRERLERLGHDVPLVGDFHYIGHKLLADHPACAEALAKYR INPGNVGFKDKKDKQFADIVEMAIRYDKPVRIGVNWGSLDQELLTTLMDRNQAEGAPLSA QDVMREAIVQSALISANLAEEIGLGRDKIILSAKVSQVQDLIAVYTMLAQRSNHALHLGL TEAGMGTKGIVASSAAMGILLQQGIGDTIRISLTPEPGGDRTREVQVAQELLQTMGFRQF VPIVAACPGCGRTTSTVFQELAQTIQEDIRRNMPLWREKYPGVEALSVAVMGCIVNGPGE SKHADIGISLPGTGETPSAPVFVDGKKVTTLRGPGIAEDFQKMVADYIENRFGLGRKIAS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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