Request QuoteCatalog Number: xP506553WBPSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)

Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP506553WBPYeast1mgQuote
EP506553WBPE. coli1mgQuote
BP506553WBPBaculovirus200ugQuote
MP506553WBPMammalian Cell200ugQuote

Protein Information

SpeciesWolbachia sp. subsp. Drosophila simulans (strain wRi)
UniProt IDC0R4T5
Gene NameispH; Locus:WRi_012440
Protein Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region Expressed1-309
Expression Tag6xHis
Purity>90%
AA SequenceMEIILAEPRGFCAGVKRAVDILAITLEKYKNERQVYVLHEIVHNKYIVEDFKKQGVVFVS SIKEIEDNGGILIFSAHGVSKNIEDEAKRKGIQVIDATCPLVNKVHKEAKRYENSGKELI LIGHENHPEVKGIRGRVNNPITLVQTLQDVYDLKVKDPDNLSYVTQTTLSIDDTRDIIAA LKLRFPGITGPDLKDICYATQNRQNAVKKLAEIVDMVLVVGSKNSSNSNRLLDLCTARGK RAYLIDNYRCVNKSWFQGVEKIGITAGASAPDILVDELMDYLKVSMNVEVSVMPGGIIEN VQFKIPNLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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