Request QuoteCatalog Number: xP511472ENUSize: 0.2-1mg

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Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)

Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511472ENUYeast1mgQuote
EP511472ENUE. coli1mgQuote
BP511472ENUBaculovirus200ugQuote
MP511472ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZPV5
Gene NameispH; Locus:BWG_0027
Protein Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Region Expressed1-316
Expression Tag6xHis
Purity>90%
AA SequenceMQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRYVVDSLRERGAIFIEQI SEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKVHMEVARASRRGEESILIG HAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEKLSFMTQTTLSVDDTSDVIDA LRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVLVVGSKNSSNSNRLAELAQRMGK RAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQNVVARLQQLGGGEAIPLEGREENI VFEVPKELRVDIREVD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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