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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514753DJQYeast1mgQuote
EP514753DJQE. coli1mgQuote
BP514753DJQBaculovirus200ugQuote
MP514753DJQMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio salexigens (strain ATCC 14822 / DSM 2638 / NCIB 8403 / VKM B-1763)
UniProt IDC6BYK9
Gene NameispG; Locus:Desal_0734
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-358
Expression Tag6xHis
Purity>90%
AA SequenceMINRKKTRELFIGDVGIGGDNPIRVQSMCNTDTRDALSTRAQIDALAEAGCEIVRVAVPD EEAAKALPQIRKGSPVPLVADIHFDYRLALSAMEAGIDALRINPGNIGGEDKVDSVVSAA KERNVPIRIGVNGGSLDKALLAKYGGPTPQAMVESALEHVALLEKRNFYNTKISLKSSSV LNTIAAYKLLAEKVDYPQHVGITEAGTLVRGAVKSSVGLGILFWEGLGDTMRVSLTHDPV AEVGVAWEILRSLGLRERGPEIVSCPTCGRTEIELIDLAQKVEDNLRGVEDVFTVAVMGC VVNGPGEAREADIGIAGGRGLGIIFRKGEVIRKVKGDENLLPEFMKEIELFLKEKRGQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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