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Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG)

Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504693EPUYeast1mgQuote
EP504693EPUE. coli1mgQuote
BP504693EPUBaculovirus200ugQuote
MP504693EPUMammalian Cell200ugQuote

Protein Information

SpeciesExiguobacterium sp. (strain ATCC BAA-1283 / AT1b)
UniProt IDC4L3K5
Gene NameispG; Locus:EAT1b_0571
Protein Name4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Region Expressed1-373
Expression Tag6xHis
Purity>90%
AA SequenceMSEILHRSLTRPVRVGNLIIGGSNEVIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVA CPDERAADALAEIKSRINIPLVVDIHFDYKLALKAIESGVDKIRINPGNIGRREKVEAVV NAAKAKGIPIRIGVNAGSLEKQFLEKYGYPTAQGMVESAMHHVKILEDLGFYNTIISLKA SDVNLALEAYTLAAKTFDYPLHVGITESGPLFSGSLKSAAGLGAILSLGIGSTVRVSLSD DPVEEVKVAKEVLKSFGLAANAATLISCPTCGRIEIDLISIAKEVEEYIQNINVNIKVAV LGCAVNGPGEAREADIGIAGARNEGLLFRHGKIIRKVPEATMVEELKKEIDAIAAEKMAE REREEQELANQSN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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