HTLV-1 Envelope Recombinant
The E. Coli derived recombinant protein contains the C- terminus of gp46 and most of p21E of HTLV-1.This non-fusion, E. coli-derived protein, starts from HIV-1 env. Amino acid 165, and is ending in amino acid 440, Mw 27 kDa.
Formulation
10mM Na-PO4 pH 6.0, 0.1% SDS and 1mM DTT.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purification Method
HTLV-1 Envelope was purified by proprietary chromatographic technique.
Purity
HTLV-1 Envelope protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
Solubility
It is recommended to reconstitute the lyophilized HTLV-1 Envelope in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Specificity
Immunoreactive with all sera of HTLV-I and HTLV-II infected individuals with antibody response to HTLV envelope.
Storage
Lyophilized HTLV-1 Envelope although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HTLV-1 Envelope should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
