$148.20Catalog Number: MAB307-20ugSize: 20ug

HSP27 Antibody

Heat Shock Protein 27 , Mouse Anti Human
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

HSP27 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50.

Clone

P2A5AT.

Ig Subclass

Mouse IgG1 heavy chain and κ light chain.

Immunogen

Anti-human HSP27 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HSP27 amino acids 1-205 purified from E. coli.

Protein formulation

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

Purification Method

HSP27 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Stability / Shelf Life

12 months at -20oC. 1 month at 4oC.

Storage Procedures

For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.

Synonyms

HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.

Type

Mouse Anti Human Monoclonal.

Quantity

$148.20

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