HMGB1 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~300. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:200.

PJ2E1AT.

Mouse IgG2b heavy chain and κ light chain.

Anti-human HMGB1 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human HMGB1 amino acids 1-215 purified from High Five (Trichoplusia ni) insect cells.

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

HMGB1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

12 months at -20^oC. 1 month at 4^oC.

For periods up to 1 month store at 4^oC, for longer periods of time, store at -20^oC. Prevent freeze thaw cycles.

HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.

Mouse Anti Human Monoclonal.