$1,798.20Catalog Number: VHIV146-100ugSize: 100ug

HIV-1 gp120 LAV

HIV-1 gp120 LAV Envelope Recombinant

HIV-1 gp120 LAV isolate Recombinant- is the external envelope protein, full-length 100-120 kDa, derived from the env. gene of HIV-1 and glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Purified under conditions that maintain the tertiary structure of the biologically active molecule. HIV-1 gp120 LAV sequence is identical to the predicted amino acid sequence of gp120 from pNL4-3 (Adachi et al. [1986], J. Virol.59, 284-291; GenBank accession number M19921).

Accession Number: M19921

Amino Acid Sequence

IPGEKLWVTV YYGVPVWKEA TTTLFCASDA KAYDTEVHNV ATHACVPTDP NPQEVVLVNV TENFNMWKND MVEQMHEDII SLWDQSLKPC VKLTPLCVSL KCTDLKNDTN TNSSSGRMIM EKGEIKNCSF NISTSIRDKV QKEYAFFYKL DIVPIDNTSY RLISCNTSVI TQACPKVSFE PIPIHYCAPA GFAILKCNNK TFNGTGPCTN VSTVQCTHGI RPVVSTQLLL NGSLAEEDVV IRSANFTDNA KTIIVQLNTS VEINCTRPNN NTRKSIRIQR GPGRAFVTIG KIGNMRQAHC NISRAKWNAT LKQIASKLRE QFGNNKTIIF KQSSGGDPEI VTHSFNCGGE FFYCNSTQLF NSTWFNSTWS TEGSNNTEGS DTITLPCRIK QFINMWQEVG KAMYAPPISG QIRCSSNITG LLLTRDGGNN NNGSEIFRPG GGDMRDNWRS ELYKYKVVKI EPLGVAPTKA KRRVVQREKR.

Applications

HIV-1 gp120 antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.

Formulation

The protein solution contains 10mM Tris-Cl pH-7.6, 150mM NaCl and 0.01% Triton N-101.

Physical Appearance

Sterile filtered colorless clear solution.

Purity

Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.

Source

Baculovirus

Specificity

Immunoreactive with sera from HIV infected individuals. Western blots: 0.1-1.0 ug/strip.

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Quantity

$1,798.20

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