Request QuoteCatalog Number: xP503873RMNSize: 0.2-1mg

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Recombinant Histidine--tRNA ligase (hisS)

Recombinant Histidine--tRNA ligase (hisS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503873RMNYeast1mgQuote
EP503873RMNE. coli1mgQuote
BP503873RMNBaculovirus200ugQuote
MP503873RMNMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia africae (strain ESF-5)
UniProt IDC3PN13
Gene NamehisS; Locus:RAF_ORF0386
Protein NameHistidine--tRNA ligase
Region Expressed1-414
Expression Tag6xHis
Purity>90%
AA SequenceMTEKLQPLRGMKDLLPDDYKVHDYIINKARDVGVLYGYKQMSTPLVEYTKVFNRSMGESS DVISKEIYSFLDKSNDFVALRPEFTACIIRSLISNRLQHKLPLKFFSTGPVFRYDRPQAG RQRQFHQLNYEYIGAKGAITDADTLKLAVDILKALEIEQDTTLELNSLGCNESRSVYQQK LVEYLNDFKDQLSEESKIRLSKNPMRILDSKSETDQKIIANAPVLSEYYTDESKEYFEEL IQYLDILGMKYSINPRLVRGLDYYCHTAFEFTTKKLGSQSTILAGGRYDGLAKIMGNNDD VPAIGFAAGIERIALMREYNISEVKPVFVLPIGKNNICYALEIVDKLRTENIAIIIESLG KIAKRMQRIFNENAQFIIFIGDEEQANNNLKIKDLKKAEEYIVDFAKALELLKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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