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Recombinant Histidine ammonia-lyase (hutH)

Recombinant Histidine ammonia-lyase (hutH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP010123XAFYeast1mgQuote
EP010123XAFE. coli1mgQuote
BP010123XAFBaculovirus200ugQuote
MP010123XAFMammalian Cell200ugQuote

Protein Information

SpeciesXanthomonas campestris pv. campestris (strain 8004)
UniProt IDQ4UTB9
Gene NamehutH; Locus:XC_2655
Protein NameHistidine ammonia-lyase
Region Expressed1-513
Expression Tag6xHis
Purity>90%
AA SequenceMSASVVLQPGQVTLAQWRALYRGAEVTLDPACAQAVLRSAQTVEAIVARGEPVYGVNTGF GKLASVRIERDDLQTLQRNIVLSHAAGVGDPTPVPVVRLMMALKLTSLAQGASGIQPDTL ALLEAMLRQGITPVVPCQGSVGASGDLAPLSHLAAVMIGVGEAFVGDQRLPAADALARAQ LQPRVLGAKEGLALLNGTQFSTACALAGLFEIETVLQAALVTGALSVEAAKGSDTPFDAR IHALRGQPGQIATAAALRALMAESAIRESHRLGDVRVQDPYCLRCQPQVMGAALDVMRQA ARTLEIEANGVSDNPLVFSDTGEALSGGNFHAEPVAFAADMLALAVCEIGSISERRTAML VDPALSGLPAFLTPRPGLNSGFMIPQVTAAALVSENKQCAYPASVDSIPTSANQEDHVSM AAHGARRLLAMADNAAHVIGIELLAAVQGCDFHAPLRSSAALEAARALLRAQVPTLQDDR YFHPDMLAASALVRSGALATAVGIVLPGVELSA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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