Hepatitis C Virus NS4 Mosaic Recombinant
The E. Coli derived 66kDa recombinant HCV NS4 Mosaic protein fused to a His tag is an artificial mosaic polypeptide composite constructed from diagnostically relevant antigenic regions derived from the NS4 region of different HCV genotypes. The protein contains region 5-1-1 and region 59 containing sequence 1789 - 1867 aa and sequence 2322 - 2423 aa of the HCV NS4 genotype lb and 10 other diagnostically relevant antigenic regions derived from the NS4 region of different HCV genotypes.
Applications
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Formulation
50mM Tris. pH-8.0, 5mM EDTA.
Purification Method
Purified by proprietary chromatographic technique.
Purity
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Specificity
Immunoreactive with sera of HCV-infected individuals.
Storage
HCV NS4 Mosaic although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
