Hepatitis C Virus NS4 a+b. Fluoroscein Recombinant
The E. coli derived 19 kDa recombinant protein Fluoroscien labeled contains the HCV NS4 immunodominant regions, amino acids 1658-1863. The protein is fused with b-galactosidase (114 kDa) at N-terminus.
Applications
HCV NS4 a+b Fluoroscein antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Formulation
20mM Tris-Hcl pH 8, 8M urea & 10mM B-ME.
Purification Method
HCV NS4 a+b Fluoroscein protein was purified by proprietary chromatographic technique.
Purity
HCV NS4 a+b Fluoroscein protein is >95% pure as determined by 10% PAGE (coomassie staining).
Specificity
Immunoreactive with sera of HCV-infected individuals.
Storage
HCV NS4 a+b Fluoroscein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.