1.Immunochromatography (capture and conjugate).2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS2.3. ELISA.

HBsAg protein was lyophilized from 0.2?m filtered (1mg/ml) solution in 20mM PB, pH 7.4 and 50mM NaCl.

HBsAg protein was purified by proprietary chromatographic technique.

HBsAg protein is >95% pure as determined by 10% PAGE (coomassie staining).

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

E. coli

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.