$298.80Catalog Number: BXJ1100-SSize: 100 ug

GST-cJun(1-79)

Human c-Jun Protein (1-79), Assay Kit

GST-cJun(1-79) is a recombinant fusion protein expressed in E. coli, consisting of GST(Glutathione-S-Transferase) followed by a Thrombin cleavage (refer to sequence below) and the activation domain (amino acid residues 1-79) of human c-Jun. JNK phosphorylation sites is also noted in the sequences.

Other Sizes

BXJ1100-S: 100 ug
BXJ1100-L: 10 mg

Custom size is also available upon request.

Kit Contents

GST-cJun in 25mM Tris (pH7.4) containing 0.5mM EDTA, 50mM NaCl, 1mM DTT, 10% glycerol at 1.0mg/ml
10X Kinase Buffer: 1.0ml of 250mM HEPES buffer (pH7.4) containing 100mM MgOAc, 0.5mM ATP, 10mM DTT.

Specifications

Preservative: 10% Glycerol
Molecular Weight: 35 kD
Purity: >90% on 15% SDS-PAGE
Source: Recombinant mature protein expressed in E. coli (189aa)
QC test: SDS-PAGE

Shipping and Storage

Shipped frozen on dry ice. Upon receiving, store the product at -80oC until use. If not all the content is to be used, aliquot the original vial and refreeze at 80oC after the initial thaw. Working aliquot can be stored at 2-8oC for no more than 2 weeks. Avoid repeated freezing and thawing.

Usage in Kinase Assay

GST-cJun (1-79) serves as a highly specific substrate for c-Jun N-terminal kinases (JNK) due to the presence of a JNK docking region. It's not phosphorylated by ERKs and p38 MAPKs. Therefore, it's a preferred substrate for in vitro JNK activity assays. Standard reaction conditions are performed by incubating the following mixture with various amounts of JNK at 30oC for 30 min in a total volume of 40 ul, followed by SDS-PAGE analysis. A standard reaction mixture contains: 5ul of 10X Kinase Buffer provided, 2uCi [P32]-ATP, 2ug of GST-cJun protein and various amounts of JNK (e.g. 0.1ug of recombinant JNK, cell lysates or immunoprecipitates.

Amino Acid Sequence

GST-(LVPRGS) QTAKGETTFY DDALNASFLP SESGPYGYSN PKILKQSMTL NLADPVGSLK PHLRAKNSDL LT(S)PDVGLLK LASPELERL

Quantity

$298.80

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