$90.00Catalog Number: PCM375-5ugSize: 5ug

GRO a Rat

GRO-Alpha Rat Recombinant (CXCL1)

CXCL1 Rat Recombinant produced in E.Coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7.8 kDa.
The Rat CXCL1 is purified by proprietary chromatographic techniques.

Accession

P14095

Amino Acid Sequence

APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA
CLDPEAPMVQ KIVQKMLKGV PK.

Biological Activity

Determined by its ability to chemoattract rat neutrophils using a concentration range of 10-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Formulation

Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, and 150mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized CXCL1 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized CXCL1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution CXCL1 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha(1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.

Quantity

$90.00

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