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Recombinant GRIP and coiled-coil domain-containing protein C27D7.02c (SPAC27D7.02c)

Recombinant GRIP and coiled-coil domain-containing protein C27D7.02c (SPAC27D7.02c) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522111SXVYeast1mgQuote
EP522111SXVE. coli1mgQuote
BP522111SXVBaculovirus200ugQuote
MP522111SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO42657
Gene NameORFs:SPAC27D7.02c
Protein NameGRIP and coiled-coil domain-containing protein C27D7.02c
Region Expressed1-750
Expression Tag6xHis
Purity>90%
AA SequenceMLGRLKDQLNMTLAQGQEEAKNRRRQFQEEDQLRRNNKSSNKLSQNEEDAKNMDSVVQKL NELQNNVVAFQKLLQEKTPLSSIQDLEGFREFMENLEHRYEMTVSEVRRLSHEVNDLQTD RENLKHQFEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKINSLESAQSIEQEV ISSLKDDKTVETKNDVPEVSRPSTDTIGVSSALSKKKKKRNRKNQKKKSTKQNIEATTEN DALSESISTPDIQKVAQSEDVDAEQDTVADSKEEERRDIANDDLIVNANAKEPMHHFSFT DLDTLLNWPKYVFHHHKIHLADTIPLVFLDLKPKEYTVDLETPKLVEELTKQLHVAESTL KENSEKFKQNSESLKSRVDNLNDYITKLQNEIDECRRNLLWAESSCETIREENQKNIKKL NDAESLKSRLLQSRTQMQTELDSYITSNSQLKDEITSLKQTVSESEAERKRLFSSAQEKQ LQMKETVNKLTSLQEQNNEFDRQLKEQEEDLQNKEEELTELRKLLREQTQDSQKLRLLVE QLELERQDLKQAGENHYSNLSSDYETQIKSLESSLTNSQAECVSFQEKINELNSQIDELK LKLNEANKKYQELAISFENSNVKTQSVEPDNGLSLEALKNENQTLLKNLEDSTARYEHLQ KSFKNVFNQLRKQQPSNHGRNSSVSRSSSSVEVNSKHPGSDDMLIDKEYTRNILFQFLEQ RDRRPEIVNLLSILLDLSEEQKQKLLSVKY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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