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Recombinant GMP synthase [glutamine-hydrolyzing] (guaA)

Recombinant GMP synthase [glutamine-hydrolyzing] (guaA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP009582BUFYeast1mgQuote
EP009582BUFE. coli1mgQuote
BP009582BUFBaculovirus200ugQuote
MP009582BUFMammalian Cell200ugQuote

Protein Information

SpeciesBorrelia burgdorferi (strain N40)
UniProt IDE4QHI6
Gene NameguaA; Locus:BbuN40_B18
Protein NameGMP synthase [glutamine-hydrolyzing]
Region Expressed1-511
Expression Tag6xHis
Purity>90%
AA SequenceMNAQAILVLDFGSQYSQLIARRIREIGVYTKVIPYYTPLKEIKNMNISGIILSGSPASVY SKEAPTLDMEIFNLKIPVLGICYGMQIIVKLFGGLVSKDSKQEYGRSEIFLKDEKSLLFS ELPNKFQIIMSHGDSIEKIPDNFKQLAFTKNCIASISNETQKIYGLQFHPEVTHSEFGDQ ILKNFVFKICQAQINWSLEGNLETIVKKIKLKVGSKKVILGLSGGTDSLVCALLIKKAIN ENLICVFVNTGLLRKNENKKILELKHQYDLNIKYIDASTKFLNRLKNISDPEEKRKIIGK EFVDVFEKITLEDQNIEYLAQGTIYSDVIESKSKDSSSSKIKSHHNVGGLPDKMSLKLLE PLNEFFKDEIIQIGINLGIKKESLYRHPFPGPGLAIRIIGEVTQEKINILQEADNILTEE LFINDLYYQIRQAFVVLLPVKSVGVMGDQRTYEYTAVIRCVNTQDFMTAEWTELPYSFLK KVSSRIINEVRGINRVCYDISSKPPSTIEWE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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