$90.00Catalog Number: PYT401-2ugSize: 2ug

GM CSF Porcine

Granulocyte Macrophage-Colony Stimulating Factor Porcine Recombinant

Granulocyte Macrophage Colony Stimulating Factor Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14381 Dalton.
GM-CSF Porcine Recombinant is purified by proprietary chromatographic techniques.

Accession

Q29118

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Thr-Arg-Pro.

Biological Activity

The ED50 range= 15-30ng/ml corresponding to a Specific Activity of 33,334-66,667IU/mg, determined by the dose dependent proliferation of TF-1 cells.

Formulation

GM-CSF Porcine was lyophilized with no additives.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

GMCSF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.977 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of GM-CSF as a Reference Standard.

Purity

Greater than 95.0% as determined by:
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution GMCSF should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim.

Quantity

$90.00

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