Request QuoteCatalog Number: xP001446EORSize: 0.2-1mg

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Recombinant Glycogen debranching enzyme (glgX)

Recombinant Glycogen debranching enzyme (glgX) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001446EORYeast1mgQuote
EP001446EORE. coli1mgQuote
BP001446EORBaculovirus200ugQuote
MP001446EORMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
UniProt IDB7LSE2
Gene NameglgX; Locus:EFER_3408
Protein NameGlycogen debranching enzyme
Region Expressed1-658
Expression Tag6xHis
Purity>90%
AA SequenceMTQLTTGKATPHGATYDGHGVNFTLFSAHAERVELCVFDAQGNEVRFDLPGRSGDVWHGY LADARPGLRYGYRVHGPWQPAAGHRFNPAKLLIDPCARRVEGDLHDNPLFHAGYETPDSR DNATIAPKCVVVSDSYNWEDDAPPRTPWGNTVIYEAHVKGLTYQHPEIPAEIRGRYKALG HPVMINYFKRLGITALELMPVIHFASEPRLQRLGLTNYWGYNPMAMFALHPVYACSPESA LDEFRDAVKELHKAGIEVILDIVINHSAELDLDGPMFSLRGIDNRSYYWIKEDGDYHNWT GCGNTLNLSNPGVMEYALECLRYWVETCHVDGFRFDLASVMGRTPAFRQDAPLFTAIQNC PVLSQVKLIAEPWDIGEGGYQVGNFPPLFAEWNDHFRDATRRFWLQRNLSLGDFAGRFAG SSDVFNRNGRLPGASINLVTAHDGFTLRDCVCFTNKHNEANGEENRDGSNNNHSNNHGKE GLGGSLDLIERRRDSVHALLTTLLLSQGTPMLLAGDEHGHSQHGNNNAYCQDNALTWLDW ERANNGLTDFTAALIHLRQQIPALTRNQWWQENDGNVRWLNKDGQVLSVEEWQHGMTCMQ IMLSDRWLIALNATPHLVDIVLPEGEWRAIPPFAGEDNPVVLEVWHGSAHGLCVFQKS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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