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Recombinant Glycine dehydrogenase [decarboxylating] (gcvP)

Recombinant Glycine dehydrogenase [decarboxylating] (gcvP) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP009493ESQYeast1mgQuote
EP009493ESQE. coli1mgQuote
BP009493ESQBaculovirus200ugQuote
MP009493ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6D8X1
Gene NamegcvP; Locus:PC1_0624
Protein NameGlycine dehydrogenase [decarboxylating]
Region Expressed1-956
Expression Tag6xHis
Purity>90%
AA SequenceMTQTLSQLEHDGAFIERHIGPSVSQQQHMLSVVGATSLDALIRQIVPQDIQLPSPPAVGE AATEHEALSELKAIAGRNQRYKSYIGMGYSAVLMPPVILRNVLENPGWYTAYTPYQPEVS QGRLEALLNFQQVTQDLTGLDLASASLLDEATAAAEAMAMAKRISKLKQAERFFVADDVH PQTLDVVRTRAETFGFEIVVGKAEDALKDDAVFGVLLQQVGTTGELHDYSDLMAALKARK VVSCVASDIMALVLLTAPGKQGADIVFGSAQRFGVPMGYGGPHAAFFACRDEHKRAMPGR IIGVSRDAAGNTALRMAMQTREQHIRREKANSNICTSQVLLANIAGMYAVFHGPEGLKRI AGRIHRLTDILAAGLTQGGLLLRHRSWFDTLTIEVADKDAVLSRALSFGINLRSDLASAV GITLDEATTREDVLALFAVLLGDDHGLDIGALDAAISQQAATIPAGLLRQDAILSHPVFN RYHSETEMMRYLHRLARKDLALNQAMIPLGSCTMKLNAAAEMLPITWPEFAELHPFCPPE QALGYRQMIEQLSGWLVQLTGYDAICMQPNSGAQGEYAGLLAIRRYHESRNEAGRHLCLI PSSAHGTNPASAQMAGMDVVVVACDKQGNIDLHDLREKAQAAGEQLSCIMVTYPSTHGVY EETIREVCQIVHQYGGQVYLDGANMNAQVGITTPGYIGADVSHLNLHKTFCIPHGGGGPG MGPIGVKAHLAPFVPGHQVVKIDGVLTEQGAVSAAPFGSASILPISWMYIRMMGAEGLKQ ASQMAILNANYIATRLQQAYPVLYTGRDGRVAHECILDIRPLKESTGISEMDIAKRLIDY GFHAPTMSFPVAGTLMVEPTESESQVEIDRFVDAMLAIRAEINRVAQGEWPLDDNPLVNA PHTQAELVADWAHPYSRELAVFPAGSEHKYWPSVKRLDDVYGDRNLFCSCVPMSDY
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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