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Recombinant Glycine dehydrogenase [decarboxylating] (gcvP)

Recombinant Glycine dehydrogenase [decarboxylating] (gcvP) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP009493BWTYeast1mgQuote
EP009493BWTE. coli1mgQuote
BP009493BWTBaculovirus200ugQuote
MP009493BWTMammalian Cell200ugQuote

Protein Information

SpeciesBrucella melitensis biotype 2 (strain ATCC 23457)
UniProt IDC0RLN1
Gene NamegcvP; Locus:BMEA_B0701
Protein NameGlycine dehydrogenase [decarboxylating]
Region Expressed1-932
Expression Tag6xHis
Purity>90%
AA SequenceMTEFLPFVARHIGPRHEDERAMLAALGLPSMETLITQAVPASIRLNRALNLPAALSEADA LAELGTIMGRNVVKKSFIGAGYHGVHTPPVIQRNLFENPAWYTAYTPYQSEISQGRLELL FHFQTLVAELTGLPVACASLLDEATAVAEAIGVACRHHRDKRSRILLAGELHPQTVDVVN TRAEPLGWEIATGSDVDDNTAAIVVPWPDTRGVYGDFAKVIADAKAKGALVIAVADPLAL TIMEAPARWGADMAVGSMQRYGVPMGFGGPHAAYLAVSEALTRIIPGRIVGQSVDAHGRA AYRLALQTREQHIRRDKATSNICTAQALLANMAAAFAIWHGPAGLQAIATRVAALAARFA AALKAAGVEIAGESLFDTVTAKVPGKAAAIAAEADKGGRLIRIIDADTVGVTFDETSTEE DLTALASLFGAKPVGGDTVLVPGKERGEGFLTQEVFHSHRSETEMMRFLRRLADKDLALD RAMIPLGSCTMKLNAAAEMMPVSWNTVANLHPFAPAEQVQGYAKMTSDLEAWLCEITGFA GVSLQPNAGSQGEYAGLMAIRHYHQARGQGHRNICLIPSSAHGTNPASASMAGMSVVVVN CRPDGDIDIDDLKAKAEKHRDNLAAFMITYPSTYGVFEEGIKAFCEIVHDNGGQVYFDGA NLNALVGLARPADIGADVCHMNLHKTFCIPHGGGGPGVGPIGVAKHLVPYLPGHVEAGSE HAVAAAQFGSASILVITWMYIRMMGGAGLKKATEAAILNANYIAHRLKGVYPILYTGAHD RVAHECIVDTRVLKDSAGITVEDVAKRLIDYGFHAPTMSWPVAGTLMIEPTESEPKLEID RLCDAMIAIAGEAKKVADGVWPADDNPLANAPHTASDTLATEWKHPYTREEAVFPGGAFD PTAKYWPPVSRVDNVGGDRNLICSCPPVAAYG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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