Request QuoteCatalog Number: xP340836TNTSize: 0.2-1mg

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Recombinant Glutamate--tRNA ligase (gltX)

Recombinant Glutamate--tRNA ligase (gltX) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340836TNTYeast1mgQuote
EP340836TNTE. coli1mgQuote
BP340836TNTBaculovirus200ugQuote
MP340836TNTMammalian Cell200ugQuote

Protein Information

SpeciesThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
UniProt IDP27000
Gene NamegltX; Locus:TTHA0438
Protein NameGlutamate--tRNA ligase
Region Expressed1-468
Expression Tag6xHis
Purity>90%
AA SequenceMVVTRIAPSPTGDPHVGTAYIALFNYAWARRNGGRFIVRIEDTDRARYVPGAEERILAAL KWLGLSYDEGPDVGGPHGPYRQSERLPLYQKYAEELLKRGWAYRAFETPEELEQIRKEKG GYDGRARNIPPEEAEERARRGEPHVIRLKVPRPGTTEVKDELRGVVVYDNQEIPDVVLLK SDGYPTYHLANVVDDHLMGVTDVIRAEEWLVSTPIHVLLYRAFGWEAPRFYHMPLLRNPD KTKISKRKSHTSLDWYKAEGFLPEALRNYLCLMGFSMPDGREIFTLEEFIQAFTWERVSL GGPVFDLEKLRWMNGKYIREVLSLEEVAERVKPFLREAGLSWESEAYLRRAVELMRPRFD TLKEFPEKARYLFTEDYPVSEKAQRKLEEGLPLLKELYPRLRAQEEWTEAALEALLRGFA AEKGVKLGQVAQPLRAALTGSLETPGLFEILALLGKERALRRLERALA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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